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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Verified applications
Western blot (WB), immunodifusion (ID)
Connected products
IMS09-038-335 Fibrinogen | labelled with fluorescien
Recommended dilutions for use
1: 5000 (WB)
Molecular weight (expected | аpparent)
75 kDa
Verified reactivity
human
Possible reactivity
bovine, mouse, porcine, rabbit, rat
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
Supplementary information
only one precipitation arc in immunodiffusion and immunoelectrophoresis against normal human plasma or human fibrinogen
References
Kinzebach et al. (2013). Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol. 2013 Oct 30;14(1):48.
Scientific context
Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N-terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel et al. 2000; Rapold et al. 1989).
Notes
None
Protein number
Q9UE34
TAIR number
Refer to NCBI
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
