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Rat FGa(Fibrinogen Alpha) ELISA Kit

General information

Catalog number
ELK5747
Name
Rat FGa(Fibrinogen Alpha) ELISA Kit
Price
608.00 EUR
Size
96T

Detailed information

Alternative Names
Fib2; FG-A; Fibrinopeptide A
Assay Type
Sandwich
Sensitivity
0.57ng/mL
Standard
100ng/mL
Detection range
1.56-100ng/mL
Assay length
3h
Research Area
Metabolic pathway;Hematology;
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Alpha (FGα). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Alpha (FGα). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Alpha (FGα), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Alpha (FGα) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Description
The FGa(Fibrinogen Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus