Fibrino Gens

Welcome on Goat anti Rat fibrinogen page

Goat anti Rat fibrinogen

General information

Catalog number
GARa/Fbg
Name
Goat anti Rat fibrinogen
Price
209.00 EUR
Size
1 mL

Detailed information

Category
Primary Antibodies
Long description
The reactivity of the antiserum is restricted to rat fibrinogen molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of fibrinogen in rat plasma or other body fluids or to determine its concentration.
Antibody come from
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Other description
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added as it may interfere with the antibody activity. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Clone
Polyclonal
Antigen-antibody binding interaction
Goat anti Rat fibrinogen Antibody
Antibody is raised in
Goat
Antibody's reacts with
Rat
Antibody's reacts with these species
The antiSerum does not cross-react with any other component of Rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Antibody's specificity
Precipitating polyclonal Goat antiSerum to Rat fibrinogen.
Research interest
Veterinary
Application
Immunoprecipitation
Antibody's suited for
Immunoprecipitation. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Precipitin titre 1:32 when tested against pooled normal rat plasma in agar-block immunodiffusion titration.
Storage
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
Relevant references
no information yet
Protein number
see ncbi
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Latin name
Capra aegagrus hircus,Rattus norvegicus
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.