For research use only. Not for diagnostic procedures.
Assay Type
Sandwich
Shipping Conditions
Ice packs
Storage Temperature
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Monkey FG (Fibrinogen) ELISA Kit before 6 months
Specificity
NA
Cross Activity
No significant cross-reactivity or interference was observed.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey FBG . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey FBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey FBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey FBG. The concentration of Monkey FBG in samples can be calculated by comparing the OD of the samples to the standard curve.
Stability
NA
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
Cancer, Cardiovascular, Metabolism
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.