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Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit

General information

Catalog number
EKE61173
Name
Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit
Price
759.00 EUR
Size
96 Tests

Detailed information

Estimated Turnaround Time
6-11 business days
Target Name
NA
Target's alterntive name
TAFI
Species Reactivity
Human
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
5-320 ng/mL
Sensitivity
3 ng/mL
Precision
NA
Detection Method
Colorimetric
Assay Time
4.5 hours
Application
For research use only. Not for diagnostic procedures.
Assay Type
Sandwich
Shipping Conditions
Ice packs
Storage Temperature
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit before 6 months
Specificity
NA
Cross Activity
No significant cross-reactivity or interference was observed.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TAFI . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAFI and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TAFI. The concentration of Human TAFI in samples can be calculated by comparing the OD of the samples to the standard curve.
Stability
NA
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
Cardiovascular, Metabolism, Signal transduction
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.