Fibrino Gens

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Rat Ficolin 1 (FCN1) CLIA Kit, 96 well plate

General information

Catalog number
EKU09789
Name
Rat Ficolin 1 (FCN1) CLIA Kit, 96 well plate
Price
1092.00 EUR
Size
96T

Detailed information

Verified reactivity
Rattus norvegicus (Rat)
Detection limits
0.781-50 ng/mL
Sensitivity limit
0,03 ng/mL
Samples to be analyzed
Serum, plasma and other biological fluids
Assay duration
2 hours, 40min
Verified applications
CLIA
Assay class
Sandwich
ELISA detection
Chemiluminescent
Protein number
Q9WTS8
Alternate protein number
Please refer to SwissProt
Gene number
Please refer to GenBank
Gene name
Ficolin 1
Alternate gene name
FCNM; Collagen/fibrinogen domain-containing protein 1; Ficolin-alpha; Ficolin-A; M-ficolin
Estimated production time
7-11 business days
Shipping requirements
Blue ice
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for
8 months
ELISA's specificity
This assay has high sensitivity and excellent specificity for detection of Ficolin 1 (FCN1). No significant cross-reactivity or interference between Ficolin 1 (FCN1) and analogues was observed.
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle
The microplate provided in this kit has been pre-coated with an antibody specific to Ficolin 1 (FCN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ficolin 1 (FCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ficolin 1 (FCN1) level in the sample or standard.;
Protocol
Please see ELISA's datasheet, otherwise contact us
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ficolin 1 (FCN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ficolin 1 (FCN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area
Metabolic pathway;Infection immunity;Bone metabolism;
Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes
For research use only. Not for diagnostic procedures.
Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus